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Detection

CHROMOGENS

We offer a large variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a colored end-product that can be visualized under bright-field microscopy. 

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Biocare’s chromogens generate a broad spectrum of colors, to find out more click on the button below.

MACH DETECTION KITS

  • Biotin-free micro-polymer technology

  • Superior antigen access

  • Unsurpassed specificity and sensitivity

  • Greater primary antibody dilutions

  • Minimal background staining

To find out more on Biocare's detection solutions click on the button below.

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MACH 4™

MACH 4 is a highly sensitive universal detection system. The MACH 4 system can detect either mouse or rabbit antibodies, and is a two-step universal detection method. MACH 4 is available in a universal (anti-mouse and anti-rabbit) format, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP). MACH 4 detection consists of two reagents: the secondary, also known as the enhancing reagent, is applied between the primary antibody and micro-polymer reagent. MACH 4 is ideal for use with antibody solutions prepared from concentrates or prediluted antibody solutions. Due to its increased sensitivity, MACH 4 enables a significantly higher dilution of concentrated antibodies compared to other polymer-based detection systems.

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  • Enhanced probe to increase sensitivity 10- to 20- fold

  • Increased density of enzymes bound to tertiaries\

  • 10 – 20 times more sensitive than conventional dextran polymer systems

  • 20 – 40 times more sensitive for nuclear staining

  • Suitable to detect mouse specific anti-biotin or antidigoxigenin antibody in in situ hybridization (ISH)

  • Micro-polymer allows superior specificity/sensitivity, nuclear access and primary antibody dilution

  • Compatible with automated immunostainers

  • Compact structure enables 40-fold increase in primary antibody dilution.

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MACH 3™

MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies. Biocare offers MACH 3 Detection Kits for either mouse or rabbit primary antibodies, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

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  • A sensitivity-enhancing secondary reagent

  • Increased density of enzymes bound to tertiary target

  • 5- to 10- fold increased staining sensitivity when compared to conventional dextran polymer detection kits

  • Superior staining for nuclear and cytoplasmic/cell surface antigens

  • Elimination of background

  • High primary antibody dilution significantly improves specificity and reduces cost

  • Adapted for all Biocare’s prediluted antibodies

  • Compatible with automated immunostainers

  • MACH 3’s superior sensitivity allows clean and strong staining, even at higher primary antibody dilution factors.

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MACH 2™

MACH 2 Detection is a one-step / one-solution, biotin-free detection system which combines superior work flow and sensitivity for mouse and rabbit primary antibodies.

MACH 2 detection consists of a single reagent applied after the primary antibody. MACH 2 is ideal for use with prediluted antibodies or concentrates with equal success. MACH 2 may be used for 7-step multiplex stains with certain antibodies.

MACH 2 increases staining specificity compared to conventional dextran backbone polymer. MACH 2 is available in anti-mouse, anti-rabbit, and ‘universal’ (anti-mouse & anti-rabbit) formulations, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

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  • Increase specificity 3-4 fold in nuclear staining & 2 fold in cytoplasmic/cell surface staining

  • Superior to dextran backbone polymers

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MACH 1™

The MACH 1 Universal Polymer Detection system offers a cost-effective alternative method of polymer detection for IHC laboratories (not available in the U.S.). Specifically titered for Biocare prediluted antibodies, MACH 1 provides ­superior staining and a reduced protocol time as compared to the conventional ABC ­system.

This new two-step, biotin-free technology uses a specific probe to detect mouse antibodies. The probe is followed by an HRP-polymer that binds to both the probe and to rabbit antibodies. Time consuming avidin-biotin and normal serum blocking steps are eliminated. The innovative HRP-polymer technology provides superior staining for mouse and rabbit antibodies when compared to ABC, biotin-streptavidin (BSA) and other polymer detection kits.

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  • Elimination of avidin-biotin and normal serum blocking steps

  • Reduce protocol length by approximately 90 minutes compared to ABC method

  • High value, cost-effective alternative to biotin-streptavidin or ABC detection

  • Eliminates endogenous non-specific background staining

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Gateway Office Park

Building 1, 747 Lytton Rd
Murarrie QLD 4172

​Phone: Free Call 1800 788 498
Fax: 1300 307 595  

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