BREAK APART FISH PROBES FOR DETECTING GENE TRANSLOCATIONS
Translocations make up nearly 75% of genetic abnormalities exhibited in cancer cells.1
Certain cancers can even be characterized by shuffling of a single hallmark gene.2 For example, NTRK3 is rearranged in over 90% of infantile fibrosarcomas, and ERG transocations are found almost exclusively in prostate tumors.3,4 Displaced genes are subject to different regulatory neighbors, and may fuse with new partners to create novel fusion genes, both of which can result in abnormal gene expression, and, consequently, lead to cancer.2 Empire Genomics’ break apart FISH probes can quickly and reliably map such rearrangements by hybridizing to the regions on either side of a target gene. A normal sample will display a unified signal, but if the gene has moved from its typical location, the two colors will split. Listed below are some of the most popular break apart probes.
Don’t see the probe you’re looking for? Contact us for a custom order - Empire Genomics can design a probe to hybridize to virtually any human gene!
1. Futreal PA, et al. Nature rev canc (2004) 4.3: 177. 2. Edwards PA. Jour Pathol (2010) 220.2: 244-254. 3. Cocco E, et al. (2018) Nature Reviews Clinical Oncology 15.12: 731-747. 4. Scheble VJ, et al. (2010) Modern Pathology 23.8: 1061
Break Apart FISH Probes
Break-apart probes are designed to detect translocations, the most common genetic abnormality exhibited in cancer cells. With new gene rearrangements being continuously discovered, and their oncogenic effects elucidated, translocations have become essential cancer biomarkers.
Our dual-colored break-apart FISH probes have helped thousands of researchers quickly and reliably track gene translocations. They’re designed to flank either side of a gene, so that in the event of a translocation, the two colors will split. Each kit contains 20 tests and normally ships within 1-3 weeks. These probes are FISH confirmed on normal peripheral blood in both interphase nuclei and metaphase spreads. These products are for research use only (RUO) and are not for use in diagnostic procedures.